GRAND CHALLENGES IN MASS SPECTROMETRY

GRAND CHALLENGES IN MASS SPECTROMETRY
Cooks R.G.
Department of Chemistry, Purdue University, West Lafayette, IN, USA
Three major challenges for molecular mass spectrometry are presented and it is argued
that each of these is achievable on a five-year time scale. These objectives are:
1. Future mass spectrometers will see routine use in surgical diagnosis and clinical pointof-
care applications. There is already considerable progress towards ambient ionization for
tissue diagnostics using complex lipid profiles, especially in brain cancer [1]. Applications
in clinical POC are emerging rapidly [2]. Both types of applications call for small, portable
instruments [3].
2. Future mass spectrometers will operate at atmospheric pressure. Ambient ionization
[4], ambient ion focusing [5], and ambient ion detection [6] are already established. The
ability to make mass/charge measurements without vacuum is still to be realized.
3. Future mass spectrometers will find routine use in organic and nanomaterial synthesis.
Preparative mass spectrometry is already used to modify surfaces through the method of
ion soft landing [7]. More recently electrolytic spray from an inert solvent has been shown
to be a method of creating nanomaterials [8] while the remarkable acceleration of chemical
reactions in microdroplets [9] has been used to synthesize organic compounds on the mg
scale.
References
1. Jarmusch A.K., Pirro V., Baird Z., et al. PNAS, 113, 1486-1491 (2016)
2. Ferreira C.R., Yannel K.E., Jarmusch A.K., et al. Clin. Chem. 62 99-110 (2016)
3. Snyder D.T., Pulliam C.J., Ouyang Z., et al. Anal. Chem. 88 2-29 (2016)
4. Monge M.E., Harris G.A., Dwivedi P., Fernandez F.M. Chem. Rev. 113, 2269 – 2308 (2013)
5. Baird Z., Wei P., Cooks R.G., Analyst, 140 696-700 (2015)
6. Hadjar O., Wang P., Futrell J.H., Dessiaterik Y., Zhu Z., Cowin J.P., Iedema M.J., Laskin J. Anal.
Chem.,79, 6566–6574 (2007)
7. Cyriac J., Pradeep T., Kang H., Chem. Rev. 112, 5356 – 5411 (2012)
8. Li A., Baird Z., Bag S., et al. Angew. Chem.Int. Ed. 53 12528-12531 (2014)
9. Li Y., Yan X., Cooks R.G. Angew.Chem. Int. Ed. 55 3433-3437 (2016)
Пленарные доклады 9
NEW GENERATION PNA ANALOGUES FOR EFFECTIVE CELL PERMEATION
Ganesh K.N., Elipilli S., Kulkarni P.
Indian Institute of Science Education and Research, Dr. HomiBhabha Road,
Pune 411008, India, e-mail: kn.ganesh@iiserpune.ac.in
Peptide nucleic acids (PNA) are a class of DNA mimics, wherein the non-ionic
ethylenediamine-glycine backbone replaces the anionic sugar-phosphate backbone. They
bind complementary DNA or RNA with high affinity and selectivity and hence promised
great potential as antisense therapeutic agents. However their poor aqueous solubility,
cell penetration abilities and equal affinity for iso-sequential DNA / RNA (which decreases
their target specificity by half) have restricted their applications. In order to overcome
these drawbacks, we have designed, synthesized and evaluated several cationic, fluorinated
and gem dimethyl substituted PNAs as new generation PNA analogues that have a good
compromise of hydrophobic/hydrophilic balance for effective cell permeation. This lecture
presents a comparative study of complementation with iso-sequential DNA / RNA and cell
permeation abilities enhanced by their inherent abilities to form nanoparticles.
References
1. Jain, D.R. Jain; Ganesh, K.N. J. Org. Chem. 2014, 79, 6708−6714.
2. Jain, D.R.; Libi, A.V.; Lahiri, M.; Ganesh, K.N.J. Org. Chem. 2014, 79, 9567-9577.
3. Ellipilli, S.; Ganesh, K.N. J. Org. Chem. 2015, 80, 9185-9191.
4. Ellipilli, S.; Murthy, R.S.; Ganesh, K.N. ChemCommun, 2016, 52, 521-524.

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